Hotspot

rps15

SSC · 126367-126639 · 273 bp

Pi 0.00483

MarkerSeek score 25.70

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 3
pi: 0.00483
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	AGACTAGGCATGGTGTGGAT	45.0	50.0	58.1	58.1	1067 bp	1.0	79.7
rps15_p2	AGTCTGCGCACCAATATCTG	AGACTAGGCATGGTGTGGAT	50.0	50.0	58.1	58.1	1379 bp	1.0	79.5
rps15_p3	TTCCCAATTGTTCTGGTGCA	AGACTAGGCATGGTGTGGAT	45.0	50.0	57.9	58.1	1263 bp	1.0	79.4
rps15_p4	CTTGTAGCTGGTGCAGTCAT	AGACTAGGCATGGTGTGGAT	50.0	50.0	57.9	58.1	1012 bp	1.0	79.4
rps15_p5	TAGCTGGTGCAGTCATAGGT	AGACTAGGCATGGTGTGGAT	50.0	50.0	58.1	58.1	1008 bp	1.0	79.2