Hotspot

ndhF-rpl32

SSC · 115436-116263 · 828 bp

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Pi 0.00312
MarkerSeek score 40.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
828
variable_sites
9
pi
0.00312
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA TCTTTTTGGATATAGAAGTACGT 42.9 30.4 57.9 53.0 906-928 bp 0.928571 38.1
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA TCTTTTTGGATATAGAAGTACGTT 42.9 29.2 57.9 53.8 906-928 bp 0.928571 38.1
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA TTCTTTTTGGATATAGAAGTACGT 42.9 29.2 57.9 53.8 907-929 bp 0.928571 38.1
ndhF-rpl32_p4 ACAAAAGGTATGATCCACGCA TCTTTTTGGATATAGAAGTACGTTT 42.9 28.0 57.9 54.6 906-928 bp 0.928571 38.1
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGCA TTCTTTTTGGATATAGAAGTACGTT 42.9 28.0 57.9 54.6 907-929 bp 0.928571 38.1