Hotspot

psbE-petL

LSC · 67415-68692 · 1278 bp

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Pi 0.00279
MarkerSeek score 50.60
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1278
variable_sites
13
pi
0.00279
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1469-1479 bp 1.0 72.8
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1392-1402 bp 1.0 72.0
psbE-petL_p3 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCA 45.0 50.0 58.2 57.1 1478-1488 bp 1.0 71.9
psbE-petL_p4 TGCTGACGAATAACCAACCC GTCGTATCTTGCTCAGACCA 50.0 50.0 58.2 57.1 1483-1493 bp 1.0 71.8
psbE-petL_p5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1477-1487 bp 1.0 71.7