Hotspot

ycf4-cemA

LSC · 62808-63722 · 915 bp

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Pi 0.00251
MarkerSeek score 44.90
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
915
variable_sites
7
pi
0.00251
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT GAGAAATCCACCAGGGCAAA 45.0 50.0 58.1 58.1 1021-1027 bp 1.0 79.6
ycf4-cemA_p2 ACAAAAAGCTGCGGAATTGG GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 1037-1043 bp 1.0 79.2
ycf4-cemA_p3 GCCATTCCCTTGACTCGTAC GAGAAATCCACCAGGGCAAA 55.0 50.0 58.3 58.1 1087-1093 bp 1.0 77.6
ycf4-cemA_p4 TTGGCCTATTTCTTGCGTGT AGAAATCCACCAGGGCAAAA 45.0 45.0 58.1 57.6 1020-1026 bp 1.0 77.1
ycf4-cemA_p5 TGCGGAATTGGCCTATTTCT GAGAAATCCACCAGGGCAAA 45.0 50.0 57.6 58.1 1028-1034 bp 1.0 76.9