Hotspot

trnF-GAA-ndhJ

LSC · 50961-51669 · 709 bp

Back to Schima

Pi 0.0026
MarkerSeek score 49.80
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
709
variable_sites
6
pi
0.0026
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 846-856 bp 1.0 81.1
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 810-820 bp 1.0 80.7
trnF-GAA-ndhJ_p3 CTGGTAGAGCAGAGGACTGA AGTTGGATAGGATGGCCCTT 55.0 50.0 57.9 58.1 834-844 bp 1.0 80.0
trnF-GAA-ndhJ_p4 AATGAAGAGGATGCATCGGG AGTTGGATAGGATGGCCCTT 50.0 50.0 57.7 58.1 873-883 bp 1.0 78.9
trnF-GAA-ndhJ_p5 GATGCATCGGGAATAGTCGG AGTTGGATAGGATGGCCCTT 55.0 50.0 58.3 58.1 864-874 bp 1.0 78.8