Hotspot

psbM-trnD-GUC

LSC · 31426-32577 · 1152 bp

Back to Schima

Pi 0.00285
MarkerSeek score 36.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1152
variable_sites
8
pi
0.00285
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
36.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC GTTCGAGCCCCGTCAGTC 45.0 66.7 55.8 60.1 1204-1221 bp 1.0 41.8
psbM-trnD-GUC_p2 AGGAACTAGAATGAACAGTGCA GTTCGAGCCCCGTCAGTC 40.9 66.7 57.4 60.1 1211-1228 bp 1.0 41.8
psbM-trnD-GUC_p3 GGAACTAGAATGAACAGTGCAG GTTCGAGCCCCGTCAGTC 45.5 66.7 57.1 60.1 1210-1227 bp 1.0 41.8
psbM-trnD-GUC_p4 GGAACTAGAATGAACAGTGCA GTTCGAGCCCCGTCAGTC 42.9 66.7 55.9 60.1 1210-1227 bp 1.0 41.8
psbM-trnD-GUC_p5 CTAGAATGAACAGTGCAGTAGC GTTCGAGCCCCGTCAGTC 45.5 66.7 56.9 60.1 1206-1223 bp 1.0 41.8