Hotspot

petN-psbM

LSC · 30198-31320 · 1123 bp

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Pi 0.00355
MarkerSeek score 56.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1123
variable_sites
16
pi
0.00355
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1285-1287 bp 1.0 68.5
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1224-1226 bp 1.0 67.0
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 1285-1287 bp 1.0 64.3
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTC 50.0 45.0 57.6 56.1 1224-1226 bp 1.0 62.8
petN-psbM_p5 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTCT 50.0 42.9 57.1 57.6 1286-1288 bp 1.0 62.6