Hotspot

trnC-GCA-petN

LSC · 29381-30107 · 727 bp

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Pi 0.00177
MarkerSeek score 46.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
727
variable_sites
5
pi
0.00177
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 806-811 bp 1.0 76.9
trnC-GCA-petN_p2 AGTTCAAATCTGGGTGTCGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.5 58.2 781-786 bp 1.0 76.0
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.4 57.6 867-872 bp 1.0 75.3
trnC-GCA-petN_p4 TATCCTTTTGGCGACATGGC AGCCCAAGCGAGACTTACTA 50.0 50.0 58.6 58.2 839-844 bp 1.0 74.8
trnC-GCA-petN_p5 AGTTCAAATCTGGGTGTCGC AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.5 57.6 842-847 bp 1.0 74.3