Hotspot

atpF-atpH

LSC · 14041-14429 · 389 bp

Back to Schima

Pi 0.00583
MarkerSeek score 31.20
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
389
variable_sites
5
pi
0.00583
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GAAAGAATCGGTTACATTTTTCA GGACTGGTTGTTGCATTAGC 30.4 50.0 53.9 57.4 459-473 bp 1.0 41.2
atpF-atpH_p2 GAAAGAATCGGTTACATTTTTCA CGGAGGGAAAAATACGAGGT 30.4 50.0 53.9 57.3 524-538 bp 1.0 41.1
atpF-atpH_p3 GAAAGAATCGGTTACATTTTTCA GAGGCGGAGGGAAAAATACG 30.4 55.0 53.9 58.7 528-542 bp 1.0 41.1
atpF-atpH_p4 GAAAGAATCGGTTACATTTTTCA GCGGAGGGAAAAATACGAGG 30.4 55.0 53.9 58.7 525-539 bp 1.0 41.1
atpF-atpH_p5 GAAAGAATCGGTTACATTTTTCA GGCGGAGGGAAAAATACGAG 30.4 55.0 53.9 58.7 526-540 bp 1.0 41.1