Hotspot

trnS-GCU-trnG

LSC · 9283-9964 · 682 bp

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Pi 0.00121
MarkerSeek score 45.30
Primer available yes
Label trnS-GCU-trnG

Feature Metadata

label_name
trnS-GCU-trnG
feature_type
igs
region
LSC
length_bp
682
variable_sites
3
pi
0.00121
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG_p1 ATTAGCAATCCGCCGCTTTA AACCGAGAGACCCCTTAACT 45.0 50.0 58.0 57.7 793-794 bp 1.0 77.5
trnS-GCU-trnG_p2 CTTTAGTCCACTCAGCCACC AACCGAGAGACCCCTTAACT 55.0 50.0 57.9 57.7 778-779 bp 1.0 77.0
trnS-GCU-trnG_p3 ACGGAAAGAGAGGGATTCGA AACCGAGAGACCCCTTAACT 50.0 50.0 58.2 57.7 842-843 bp 1.0 76.6
trnS-GCU-trnG_p4 TTTAGTCCACTCAGCCACCT AACCGAGAGACCCCTTAACT 50.0 50.0 58.3 57.7 777-778 bp 1.0 75.7
trnS-GCU-trnG_p5 GCTTTAGTCCACTCAGCCAC AACCGAGAGACCCCTTAACT 55.0 50.0 58.6 57.7 779-780 bp 1.0 73.4