Hotspot

trnK-UUU-rps16

LSC · 4351-5165 · 815 bp

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Pi 0.00323
MarkerSeek score 49.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
815
variable_sites
8
pi
0.00323
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 981-990 bp 1.0 72.0
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 982-991 bp 1.0 72.0
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 986-995 bp 1.0 72.0
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.0 57.9 984-993 bp 1.0 72.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.2 57.9 932-941 bp 0.928571 68.1