Hotspot

ycf2

Genome · 30570-36638 · 6069 bp

Back to Scapania

Pi 0.07

MarkerSeek score 69.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6069
variable_sites: 423
pi: 0.07
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TTTCCCTTTAACCGCGCATA	CCCACCATTCCCATGTAAAGA	45.0	47.6	57.9	57.6	1519 bp	0.5	48.1
ycf2_p2	TTTCCCTTTAACCGCGCATA	TCCCACCATTCCCATGTAAAG	45.0	47.6	57.9	57.6	1520 bp	0.5	48.1
ycf2_p3	TTCCCTTTAACCGCGCATAT	CCCACCATTCCCATGTAAAGA	45.0	47.6	57.7	57.6	1518 bp	0.5	46.3
ycf2_p4	TTCCCTTTAACCGCGCATAT	TCCCACCATTCCCATGTAAAG	45.0	47.6	57.7	57.6	1519 bp	0.5	46.3
ycf2_p5	TTTCCCTTTAACCGCGCATA	TCCCACCATTCCCATGTAAA	45.0	45.0	57.9	56.4	1520 bp	0.5	46.2