Hotspot

atpB-rbcL

Genome · 55829-56390 · 562 bp

Back to Scapania

Pi 0.0588

MarkerSeek score 83.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 562
variable_sites: 32
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TCTTGCCAGGTGAAAAAGCA	GCTTTGAATCCAACACCTGC	45.0	50.0	58.2	57.9	702-718 bp	1.0	83.3
atpB-rbcL_p2	TCTTGCCAGGTGAAAAAGCA	GTCTCCGTTTGTGGTGACAT	45.0	50.0	58.2	58.1	678-694 bp	1.0	83.1
atpB-rbcL_p3	TTGCCAGGTGAAAAAGCAAC	GCTTTGAATCCAACACCTGC	45.0	50.0	57.7	57.9	700-716 bp	1.0	82.6
atpB-rbcL_p4	TTGCCAGGTGAAAAAGCAAC	GTCTCCGTTTGTGGTGACAT	45.0	50.0	57.7	58.1	676-692 bp	1.0	82.4
atpB-rbcL_p5	GCCAGGTGAAAAAGCAACAT	GCTTTGAATCCAACACCTGC	45.0	50.0	57.5	57.9	698-714 bp	1.0	80.9