Hotspot

trnL-UAA-trnF-GAA

Genome · 50897-50965 · 69 bp

Back to Scapania

Pi 0.101

MarkerSeek score 80.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: Genome
length_bp: 69
variable_sites: 7
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	CGTTGGCTTTTTAGACCGTG	TTTGAACTGGTGACACGAGG	50.0	50.0	57.7	58.1	172 bp	1.0	80.4
trnL-UAA-trnF-GAA_p2	TCCGTTGGCTTTTTAGACCG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.5	58.1	174 bp	1.0	78.9
trnL-UAA-trnF-GAA_p3	CGTTGGCTTTTTAGACCGTG	GAACTGGTGACACGAGGATT	50.0	50.0	57.7	57.5	169 bp	1.0	77.2
trnL-UAA-trnF-GAA_p4	CGTTGGCTTTTTAGACCGTG	TACCAACTGAGCTATCCCGG	50.0	55.0	57.7	58.6	134 bp	1.0	77.0
trnL-UAA-trnF-GAA_p5	CCGTTGGCTTTTTAGACCGT	TTTGAACTGGTGACACGAGG	50.0	50.0	58.8	58.1	173 bp	1.0	76.7