Hotspot

trnH-GUG-ycf2

Genome · 29771-30569 · 799 bp

Back to Scapania

Pi 0.094

MarkerSeek score 80.60

Primer available yes

Label trnH-GUG-ycf2

Feature Metadata

label_name: trnH-GUG-ycf2
feature_type: igs
region: Genome
length_bp: 799
variable_sites: 74
pi: 0.094
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-ycf2_p1	GCGCTAACCTTGGTATGGAA	AACCCGAGTAAACTGCGTTT	50.0	45.0	58.0	58.0	1202-1207 bp	1.0	85.9
trnH-GUG-ycf2_p2	GCGCTAACCTTGGTATGGAA	ACCCGAGTAAACTGCGTTTT	50.0	45.0	58.0	58.0	1201-1206 bp	1.0	85.9
trnH-GUG-ycf2_p3	TGTTGCAGCTCACGGTTATT	ACCCGAGTAAACTGCGTTTT	45.0	45.0	58.1	58.0	1425-1430 bp	1.0	84.4
trnH-GUG-ycf2_p4	TGGGTGTTGCTGGTGTATTC	AACCCGAGTAAACTGCGTTT	50.0	45.0	58.1	58.0	1571-1576 bp	1.0	84.0
trnH-GUG-ycf2_p5	TGGGTGTTGCTGGTGTATTC	ACCCGAGTAAACTGCGTTTT	50.0	45.0	58.1	58.0	1570-1575 bp	1.0	84.0