Hotspot

chlB-trnK-UUU

Genome · 25710-26216 · 507 bp

Back to Scapania

Pi 0.0655

MarkerSeek score 80.10

Primer available yes

Label chlB-trnK-UUU

Feature Metadata

label_name: chlB-trnK-UUU
feature_type: igs
region: Genome
length_bp: 507
variable_sites: 31
pi: 0.0655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-trnK-UUU_p1	GACAAAACGGTGTTGCCAAA	CAAGTCGCACTTAAAAGCCG	45.0	50.0	58.0	58.0	587-622 bp	1.0	87.7
chlB-trnK-UUU_p2	GACAAAACGGTGTTGCCAAA	AAGTCGCACTTAAAAGCCGA	45.0	45.0	58.0	58.1	586-621 bp	1.0	87.1
chlB-trnK-UUU_p3	CGGAAAAATTTGCCCGACAA	CAAGTCGCACTTAAAAGCCG	45.0	50.0	57.9	58.0	602-637 bp	1.0	86.5
chlB-trnK-UUU_p4	CGGAAAAATTTGCCCGACAA	AAGTCGCACTTAAAAGCCGA	45.0	45.0	57.9	58.1	601-636 bp	1.0	85.9
chlB-trnK-UUU_p5	GACAAAACGGTGTTGCCAAA	CCAAGTCGCACTTAAAAGCC	45.0	50.0	58.0	57.7	588-623 bp	1.0	85.4