Hotspot

trnR-UCU-trnG-UCC

Genome · 21524-21600 · 77 bp

Back to Scapania

Pi 0.107

MarkerSeek score 81.40

Primer available yes

Label trnR-UCU-trnG-UCC

Feature Metadata

label_name: trnR-UCU-trnG-UCC
feature_type: igs
region: Genome
length_bp: 77
variable_sites: 8
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-UCC_p1	ATCCTTTAGACGATGGACGC	ACCCTTAGCCTTCCAAGCTA	50.0	50.0	57.5	58.0	145-147 bp	1.0	81.1
trnR-UCU-trnG-UCC_p2	ATCCTTTAGACGATGGACGC	TTTGCATGTTCGGTTCTGGA	50.0	45.0	57.5	58.0	203-205 bp	1.0	79.6
trnR-UCU-trnG-UCC_p3	ATCCTTTAGACGATGGACGC	TTCCAAGCTAATGATGCGGG	50.0	50.0	57.5	58.3	135-137 bp	1.0	79.2
trnR-UCU-trnG-UCC_p4	ATCCTTTAGACGATGGACGC	TCGGTTCTGGAGTAGAGACG	50.0	55.0	57.5	58.3	194-196 bp	1.0	77.9
trnR-UCU-trnG-UCC_p5	ATCCTTTAGACGATGGACGC	GCATGTTCGGTTCTGGAGTA	50.0	50.0	57.5	57.6	200-202 bp	1.0	76.9