Hotspot

rps12-rps7

Genome · 997-1046 · 50 bp

Back to Scapania

Pi 0.106

MarkerSeek score 78.20

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: Genome
length_bp: 50
variable_sites: 5
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	AATCGGATGTACGGTTTGGG	ATTGGATCAGGCTTCGCAAT	50.0	45.0	58.0	57.9	167-171 bp	1.0	85.4
rps12-rps7_p2	AATCGGATGTACGGTTTGGG	CGTGACGTTCGGAGTTACTT	50.0	50.0	58.0	58.0	339-343 bp	1.0	83.6
rps12-rps7_p3	AATCGGATGTACGGTTTGGG	GATCCACCTATACGACGTGC	50.0	55.0	58.0	58.0	365-369 bp	1.0	83.3
rps12-rps7_p4	AATCGGATGTACGGTTTGGG	GCAGCCATATTTCCACCTGA	50.0	50.0	58.0	57.9	470-474 bp	1.0	83.0
rps12-rps7_p5	AATCGGATGTACGGTTTGGG	TTACTGATGGCTTGACGTGG	50.0	50.0	58.0	57.9	324 bp	0.5	59.0