Hotspot

ycf1

SSC · 128204-133972 · 5769 bp

Back to Scabiosa

Pi 0.0866

MarkerSeek score 71.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5769
variable_sites: 417
pi: 0.0866
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCTCAAATGATTCCCGCC	GCCCGACAAAATCTTTCGTG	50.0	50.0	58.0	58.0	1057 bp	0.5	59.6
ycf1_p2	GCAGCTTAGAACCAGTACCC	GCCCGACAAAATCTTTCGTG	55.0	50.0	58.0	58.0	541 bp	0.5	59.5
ycf1_p3	GGCAGCTTAGAACCAGTACC	GCCCGACAAAATCTTTCGTG	55.0	50.0	58.0	58.0	542 bp	0.5	59.5
ycf1_p4	GGGCAGCTTAGAACCAGTAC	GCCCGACAAAATCTTTCGTG	55.0	50.0	58.0	58.0	543 bp	0.5	59.5
ycf1_p5	ATCCTCAAATGATTCCCGCC	GAGAAAGCAAGCCCGAAAAC	50.0	50.0	58.0	57.9	994 bp	0.5	59.5