Hotspot

trnL-UAG

SSC · 116427-116506 · 80 bp

Back to Scabiosa

Pi 0.512

MarkerSeek score 72.50

Primer available yes

Label trnL-UAG

Feature Metadata

label_name: trnL-UAG
feature_type: tRNA
region: SSC
length_bp: 80
variable_sites: 41
pi: 0.512
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG_p1	TATCACGCCCTTTTCTGACG	TGAACTCATCGCATCCTGTT	50.0	45.0	58.0	57.2	440 bp	0.5	53.8
trnL-UAG_p2	TATCACGCCCTTTTCTGACG	TTGAACTCATCGCATCCTGT	50.0	45.0	58.0	57.2	441 bp	0.5	53.8
trnL-UAG_p3	GCTATCACGCCCTTTTCTGA	TGAACTCATCGCATCCTGTT	50.0	45.0	58.0	57.2	442 bp	0.5	53.7
trnL-UAG_p4	GCTATCACGCCCTTTTCTGA	TTGAACTCATCGCATCCTGT	50.0	45.0	58.0	57.2	443 bp	0.5	53.7
trnL-UAG_p5	AAAAATCGCACTTGACGCAG	TGAACTCATCGCATCCTGTT	45.0	45.0	58.0	57.2	626 bp	0.5	53.6