Hotspot

rpl32

SSC · 117311-117481 · 171 bp

Back to Scabiosa

Pi 0.47

MarkerSeek score 75.30

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 171
variable_sites: 78
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	AACAGGATGCGATGAGTTCA	AGGATTGGCGGAATTGACTC	45.0	50.0	57.2	58.0	1241 bp	0.5	53.5
rpl32_p2	AACAGGATGCGATGAGTTCA	GAGGATTGGCGGAATTGACT	45.0	50.0	57.2	58.0	1242 bp	0.5	53.5
rpl32_p3	ACAGGATGCGATGAGTTCAA	AGAGGATTGGCGGAATTGAC	45.0	50.0	57.2	58.0	1242 bp	0.5	53.5
rpl32_p4	ACAGGATGCGATGAGTTCAA	AGGATTGGCGGAATTGACTC	45.0	50.0	57.2	58.0	1240 bp	0.5	53.5
rpl32_p5	ACAGGATGCGATGAGTTCAA	GAGGATTGGCGGAATTGACT	45.0	50.0	57.2	58.0	1241 bp	0.5	53.5