Hotspot

ndhF

SSC · 117620-119887 · 2268 bp

Back to Scabiosa

Pi 0.473

MarkerSeek score 80.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2268
variable_sites: 940
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAAAGCTTTTAACGCGGACC	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.3	58.0	2755 bp	0.5	57.4
ndhF_p2	TAACGCGGACCAATATGCTT	TGGACCGCGAGAGTTATTTG	45.0	50.0	57.7	58.0	2746 bp	0.5	57.4
ndhF_p3	TTAACGCGGACCAATATGCT	TGGACCGCGAGAGTTATTTG	45.0	50.0	57.7	58.0	2747 bp	0.5	57.4
ndhF_p4	CAAAGCTTTTAACGCGGACC	GGACCGCGAGAGTTATTTGT	50.0	50.0	58.3	58.0	2754 bp	0.5	57.4
ndhF_p5	GGACCAATATGCTTTCGCCT	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.3	58.0	2740 bp	0.5	57.4