Hotspot

accD

LSC · 59803-61644 · 1842 bp

Back to Scabiosa

Pi 0.208

MarkerSeek score 78.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1842
variable_sites: 307
pi: 0.208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTTCTACCCGCGCTTTCTAC	TATACGTAAGGCGGGAGGAG	50.0	55.0	58.0	58.1	2656 bp	0.5	59.2
accD_p2	TTTCTACCCGCGCTTTCTAC	CGGGAGGAGGAAATTCGTTT	50.0	50.0	58.0	57.9	2645 bp	0.5	59.1
accD_p3	TCTACCCGCGCTTTCTACTA	TATACGTAAGGCGGGAGGAG	50.0	55.0	58.0	58.1	2654 bp	0.5	58.9
accD_p4	TCTACCCGCGCTTTCTACTA	CGGGAGGAGGAAATTCGTTT	50.0	50.0	58.0	57.9	2643 bp	0.5	58.8
accD_p5	AGTTTCCCTGGATCGAGACA	TATACGTAAGGCGGGAGGAG	50.0	55.0	58.1	58.1	2694 bp	0.5	58.7