Hotspot

rpl32-ndhF

SSC · 117482-117619 · 138 bp

Back to Scabiosa

Pi 0.349

MarkerSeek score 76.90

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 138
variable_sites: 45
pi: 0.349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CAAAGCTTTTAACGCGGACC	AGAGGATTGGCGGAATTGAC	50.0	50.0	58.3	58.0	456 bp	0.5	57.1
rpl32-ndhF_p2	CAAAGCTTTTAACGCGGACC	AGGATTGGCGGAATTGACTC	50.0	50.0	58.3	58.0	454 bp	0.5	57.1
rpl32-ndhF_p3	CAAAGCTTTTAACGCGGACC	GAGGATTGGCGGAATTGACT	50.0	50.0	58.3	58.0	455 bp	0.5	57.1
rpl32-ndhF_p4	TAACGCGGACCAATATGCTT	AGGATTGGCGGAATTGACTC	45.0	50.0	57.7	58.0	445 bp	0.5	57.1
rpl32-ndhF_p5	TAACGCGGACCAATATGCTT	GAGGATTGGCGGAATTGACT	45.0	50.0	57.7	58.0	446 bp	0.5	57.1