Hotspot

infA-rps8

LSC · 84177-84291 · 115 bp

Back to Scabiosa

Pi 0.0435

MarkerSeek score 77.50

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 5
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ACTCGGAACATACCATTGGG	CGGGAAGCTCGACTAGAAAG	50.0	55.0	57.3	57.8	237 bp	1.0	74.4
infA-rps8_p2	ACTCGGAACATACCATTGGG	TCGGCGGAGAAATTTTGTGT	50.0	45.0	57.3	58.4	215 bp	1.0	73.0
infA-rps8_p3	ACTCGGAACATACCATTGGG	CGGCGGAGAAATTTTGTGTT	50.0	45.0	57.3	57.6	214 bp	1.0	72.9
infA-rps8_p4	ACTCGGAACATACCATTGGG	AGAATTTTAGGCGGGATGGG	50.0	50.0	57.3	57.6	297 bp	1.0	72.7
infA-rps8_p5	ACTCGGAACATACCATTGGG	CGGGATGGGGATTGTCATTC	50.0	55.0	57.3	58.4	286 bp	1.0	72.6