Hotspot

ndhC-trnV-UAC

LSC · 52602-53606 · 1005 bp

Back to Scabiosa

Pi 0.0608

MarkerSeek score 86.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 58
pi: 0.0608
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1136-1204 bp	1.0	84.8
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1148-1216 bp	1.0	83.1
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1135-1203 bp	1.0	82.0
ndhC-trnV-UAC_p4	TAATCGGGGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	1141-1209 bp	1.0	81.0
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1124-1192 bp	1.0	80.9