Hotspot

atpA-atpF

LSC · 12817-12893 · 77 bp

Back to Scabiosa

Pi 0.039

MarkerSeek score 74.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 77
variable_sites: 3
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCTACTTGAAGTACGGTGCC	TTGGGAGCGATGAACGAAAT	55.0	45.0	58.0	57.9	214-219 bp	1.0	80.2
atpA-atpF_p2	CCTACTTGAAGTACGGTGCC	GGCGCTCTAGGAACTTTGAA	55.0	50.0	58.0	57.9	289-294 bp	1.0	80.0
atpA-atpF_p3	CCTACTTGAAGTACGGTGCC	AAGGCGCTCTAGGAACTTTG	55.0	50.0	58.0	57.9	291-296 bp	1.0	80.0
atpA-atpF_p4	CCTACTTGAAGTACGGTGCC	CAAGGCGCTCTAGGAACTTT	55.0	50.0	58.0	57.9	292-297 bp	1.0	80.0
atpA-atpF_p5	GAAGTACGGTGCCGGTATTT	GGCGCTCTAGGAACTTTGAA	50.0	50.0	58.0	57.9	282-287 bp	1.0	80.0