Hotspot

ycf1

SSC · 122170-125940 · 3771 bp

Back to Saxifraga

Pi 0.0592

MarkerSeek score 78.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3771
variable_sites: 1709
pi: 0.0592
unique_haplotype_count: 113
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTGTTTCGATCTGTGCTT	TGGATGCGTTTGATAGAAAATCA	45.0	34.8	58.1	56.9	692-767 bp	0.094828	21.7
ycf1_p2	TGCTGTTTCGATCTGTGCTT	TCAACAGAAAGAGACCCTTTT	45.0	38.1	58.1	55.0	732 bp	0.017241	7.5
ycf1_p3	AATCTGAGGATCCTCTGCCA	TCAACAGAAAGAGACCCTTTT	50.0	38.1	57.8	55.0	309 bp	0.017241	6.9
ycf1_p4	TGCTGTTTCGATCTGTGCTT	ATCAACAGAAAGAGACCCTT	45.0	40.0	58.1	53.8	733 bp	0.017241	6.5
ycf1_p5	ATCTGAGGATCCTCTGCCAG	TCAACAGAAAGAGACCCTTTT	55.0	38.1	58.3	55.0	308 bp	0.017241	6.2