Hotspot

ycf1-trnN-GUU

IRa · 125941-127904 · 1964 bp

Back to Saxifraga

Pi 0.0219

MarkerSeek score 65.40

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1964
variable_sites: 373
pi: 0.0219
unique_haplotype_count: 97
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	AATCTGAGGATCCTCTGCCA	ATCGGGATAGTGGACCCAAA	50.0	50.0	57.8	58.1	2726-2969 bp	0.87069	84.4
ycf1-trnN-GUU_p2	AATCTGAGGATCCTCTGCCA	GAACTTGGGTGTGGGTCTTT	50.0	50.0	57.8	57.9	2700-2917 bp	0.793103	81.7
ycf1-trnN-GUU_p3	AATCTGAGGATCCTCTGCCA	CTATGGAACTTGGGTGTGGG	50.0	55.0	57.8	57.9	2705-2922 bp	0.793103	81.2
ycf1-trnN-GUU_p4	AATCTGAGGATCCTCTGCCA	CTCTTAACAAGCGAGCCCTT	50.0	50.0	57.8	57.9	2345-2427 bp	0.043103	45.2
ycf1-trnN-GUU_p5	AATCTGAGGATCCTCTGCCA	GGGGGATCATGATCGGGATA	50.0	55.0	57.8	58.1	2737-2882 bp	0.025862	43.0