Hotspot

ndhF-rpl32

SSC · 111281-111881 · 601 bp

Back to Saxifraga

Pi 0.0672

MarkerSeek score 79.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 601
variable_sites: 290
pi: 0.0672
unique_haplotype_count: 104
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGCAATAGGTCGTGTGAACC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1447-1834 bp	1.0	91.2
ndhF-rpl32_p2	CTGAATAGCCAGAGCCACAG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.0	58.0	1355-1502 bp	0.103448	55.7
ndhF-rpl32_p3	CTGAATAGCCAGAGCCACAG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.0	58.0	1356-1503 bp	0.103448	55.7
ndhF-rpl32_p4	CTGAATAGCCAGAGCCACAG	CCCTTAACGCTGCCCAATAT	55.0	50.0	58.0	58.0	811-904 bp	0.103448	55.6
ndhF-rpl32_p5	CTGAATAGCCAGAGCCACAG	TTAACGCTGCCCAATATCCC	55.0	50.0	58.0	58.0	808-901 bp	0.103448	55.5