Hotspot

psbE-petL

LSC · 64091-65339 · 1249 bp

Back to Saxifraga

Pi 0.0445

MarkerSeek score 79.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1249
variable_sites: 437
pi: 0.0445
unique_haplotype_count: 106
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	854-1366 bp	1.0	90.7
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	856-1368 bp	1.0	90.7
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GCCGCTAGTAGAAAACCGAA	55.0	50.0	58.2	58.0	849-1361 bp	1.0	90.4
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AAGCCGCTAGTAGAAAACCG	55.0	50.0	58.2	58.0	851-1363 bp	1.0	90.4
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.4	58.0	847-1359 bp	1.0	89.2