Hotspot

ndhC-trnV-UAC

LSC · 50641-51464 · 824 bp

Back to Saxifraga

Pi 0.0695

MarkerSeek score 68.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 330
pi: 0.0695
unique_haplotype_count: 94
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAGAAGCGGCCATAAACTG	ATGAATCAGAGCGCATGGAG	50.0	50.0	58.0	58.1	838-1896 bp	1.0	90.8
ndhC-trnV-UAC_p2	AGAGAAGCGGCCATAAACTG	ACAAGCACAAGGGCTATAGC	50.0	50.0	58.0	58.0	1314-2122 bp	0.922414	88.3
ndhC-trnV-UAC_p3	AAACTGGAGAGTCTGGACCA	ACAAGCACAAGGGCTATAGC	50.0	50.0	57.9	58.0	1300-2108 bp	0.922414	87.7
ndhC-trnV-UAC_p4	AACTGGAGAGTCTGGACCAA	ACAAGCACAAGGGCTATAGC	50.0	50.0	57.9	58.0	1299-2107 bp	0.922414	87.7
ndhC-trnV-UAC_p5	AGAGAAGCGGCCATAAACTG	GAAGAGTCCACCATGCACAT	50.0	50.0	58.0	57.9	1248-2288 bp	0.913793	87.4