Hotspot

psbZ-trnG-UCC

LSC · 35727-36388 · 662 bp

Back to Saxifraga

Pi 0.0765

MarkerSeek score 64.40

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 249
pi: 0.0765
unique_haplotype_count: 77
primer_available: yes
alignment_reliability: 0.36
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	AGTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.8	57.8	301-780 bp	1.0	81.0
psbZ-trnG-UCC_p2	AGTCTTTCTGGTGGGTATCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	301-780 bp	1.0	76.5
psbZ-trnG-UCC_p3	GTCTTTCTGGTGGGTATCCT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.2	57.8	300-779 bp	1.0	76.5
psbZ-trnG-UCC_p4	AGTCTTTCTGGTGGGTATCCT	TACCACTTGACCATATCCGC	47.6	50.0	57.8	57.1	274-753 bp	1.0	75.1
psbZ-trnG-UCC_p5	TGGATTAGTCTTTCTGGTGGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.0	57.8	307-786 bp	1.0	74.6