Hotspot

trnT-GGU-psbD

LSC · 31092-32463 · 1372 bp

Back to Saxifraga

Pi 0.0554

MarkerSeek score 71.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1372
variable_sites: 499
pi: 0.0554
unique_haplotype_count: 94
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	TTACCAAGGGCTATAGTCAT	50.0	40.0	57.9	52.8	941-1561 bp	0.965517	50.6
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	TTTACCAAGGGCTATAGTCAT	50.0	38.1	57.9	53.7	942-1562 bp	0.965517	50.6
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	905-1494 bp	0.956897	50.3
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	TTTACCAAGGGCTATAGTCA	50.0	40.0	57.9	53.0	942-1531 bp	0.922414	48.9
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	ATTTACCAAGGGCTATAGTCA	50.0	38.1	57.9	53.7	943-1532 bp	0.922414	48.9