Hotspot

trnC-GCA-petN

LSC · 27781-28506 · 726 bp

Back to Saxifraga

Pi 0.047

MarkerSeek score 75.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 260
pi: 0.047
unique_haplotype_count: 96
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	412-819 bp	1.0	90.1
trnC-GCA-petN_p2	TTTAGCGAATTCACAGCCGA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	553-983 bp	0.698276	78.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AAATGAGCGGACTACAACCC	50.0	50.0	58.1	57.9	1287-1296 bp	0.025862	40.5
trnC-GCA-petN_p4	TTTAGCGAATTCACAGCCGA	AAATGAGCGGACTACAACCC	45.0	50.0	57.9	57.9	1454-1462 bp	0.025862	40.4
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	AAATGAGCGGACTACAACCC	50.0	50.0	57.9	57.9	1339-1348 bp	0.025862	40.0