Hotspot

rpoB-trnC-GCA

LSC · 26615-27701 · 1087 bp

Back to Saxifraga

Pi 0.0497

MarkerSeek score 81.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 419
pi: 0.0497
unique_haplotype_count: 108
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTTCCCCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	780-1190 bp	0.905172	86.9
rpoB-trnC-GCA_p2	ATTTCCCCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	762-1172 bp	0.905172	84.7
rpoB-trnC-GCA_p3	ATTTCCCCATTTCCATCCCG	GACACCCGGATTTGAACTGG	50.0	55.0	57.9	58.8	785-1195 bp	0.905172	80.8
rpoB-trnC-GCA_p4	ATTTCCCCATTTCCATCCCG	CGACACCCGGATTTGAACTG	50.0	55.0	57.9	59.2	786-1196 bp	0.905172	77.9
rpoB-trnC-GCA_p5	ATTTCCCCATTTCCATCCCG	CGACACCCGGATTTGAACT	50.0	52.6	57.9	57.8	786-1196 bp	0.905172	77.7