Hotspot

trnK-UUU-rps16

LSC · 4112-4943 · 832 bp

Back to Saxifraga

Pi 0.0598
MarkerSeek score 77.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
832
variable_sites
372
pi
0.0598
unique_haplotype_count
102
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 810-1081 bp 0.991379 91.3
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 809-1080 bp 0.991379 91.3
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG GAAGGGAGGGGAGAGATCTC 55.0 60.0 58.0 58.0 903-1153 bp 0.827586 84.9
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG GAAGGGAGGGGAGAGATCTC 55.0 60.0 58.0 58.0 902-1152 bp 0.827586 84.9
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA GAAGGGAGGGGAGAGATCTC 50.0 60.0 58.0 58.0 855-1105 bp 0.827586 84.7