Hotspot

rbcL

LSC · 54473-55900 · 1428 bp

Back to Saussurea

Pi 0.00366

MarkerSeek score 47.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 27
pi: 0.00366
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	GGGTTCGGCTCAATCCTTTT	50.0	50.0	57.1	58.5	1565 bp	1.0	70.3
rbcL_p2	ACGCCTAATTCATGTCGAGT	GGGTTCGGCTCAATCCTTTT	45.0	50.0	57.0	58.5	1613 bp	1.0	70.1
rbcL_p3	TCATGAGTTGTAGGGAGGGA	ATGTTGGGTTCGGCTCAATC	50.0	50.0	57.1	58.5	1570 bp	1.0	69.6
rbcL_p4	ACGCCTAATTCATGTCGAGT	ATGTTGGGTTCGGCTCAATC	45.0	50.0	57.0	58.5	1618 bp	1.0	69.4
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	GGGTTCGGCTCAATCCTTTT	47.6	50.0	57.8	58.5	1566 bp	1.0	68.2