Hotspot

ndhF-rpl32

SSC · 110918-111967 · 1050 bp

Back to Saussurea

Pi 0.00609

MarkerSeek score 61.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1050
variable_sites: 46
pi: 0.00609
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	50.0	50.0	58.0	57.6	1225-1238 bp	1.0	80.7
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1223-1236 bp	1.0	80.6
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1224-1237 bp	1.0	80.6
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1242-1255 bp	1.0	80.4
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1240-1253 bp	1.0	80.3