Hotspot

atpB-rbcL

LSC · 53715-54472 · 758 bp

Back to Saussurea

Pi 0.00226

MarkerSeek score 59.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 14
pi: 0.00226
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	887-940 bp	1.0	76.9
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	886-939 bp	1.0	76.7
atpB-rbcL_p3	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	844-897 bp	1.0	76.2
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	827-867 bp	0.958333	73.6
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	826-866 bp	0.958333	73.3