Hotspot

ycf3-trnS-GGA

LSC · 43550-44470 · 921 bp

Back to Saussurea

Pi 0.00831

MarkerSeek score 64.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 49
pi: 0.00831
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	972-1082 bp	1.0	78.4
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	922-1032 bp	1.0	78.2
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	960-1070 bp	1.0	77.9
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	924-1034 bp	1.0	77.7
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	950-1060 bp	1.0	76.5