Hotspot

trnE-UUC-rpoB

LSC · 11565-12349 · 785 bp

Back to Saussurea

Pi 0.00492

MarkerSeek score 61.60

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 32
pi: 0.00492
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GCATGATTTACCCCCAGAGG	CATTGCGTCTATCCCGGATT	55.0	50.0	58.0	57.8	903-983 bp	1.0	85.3
trnE-UUC-rpoB_p2	GGCATGATTTACCCCCAGAG	CATTGCGTCTATCCCGGATT	55.0	50.0	58.0	57.8	904-984 bp	1.0	85.3
trnE-UUC-rpoB_p3	ACCCTCGTTATCTCCGTGAA	CATTGCGTCTATCCCGGATT	50.0	50.0	58.2	57.8	876-956 bp	1.0	84.2
trnE-UUC-rpoB_p4	GCATGATTTACCCCCAGAGG	TGTGGACATTGCGTCTATCC	55.0	50.0	58.0	57.7	909-989 bp	1.0	84.1
trnE-UUC-rpoB_p5	GGCATGATTTACCCCCAGAG	TGTGGACATTGCGTCTATCC	55.0	50.0	58.0	57.7	910-990 bp	1.0	84.1