Hotspot

ndhI

SSC · 126087-126599 · 513 bp

Back to Saururus

Pi 0.446

MarkerSeek score 79.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 513
variable_sites: 198
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	ATGAAGTAACCCCATCCCCA	GCGTCAACCCATAGGCTTTA	50.0	50.0	58.0	58.0	1767 bp	0.5	59.7
ndhI_p2	CGTTTATGGCTCCCACGTAA	GCGTCAACCCATAGGCTTTA	50.0	50.0	58.0	58.0	1677 bp	0.5	59.6
ndhI_p3	CCCAACGGTCCAAAGAGAAA	GCGTCAACCCATAGGCTTTA	50.0	50.0	58.0	58.0	1751 bp	0.5	59.5
ndhI_p4	ATGAAGTAACCCCATCCCCA	TGTACTTGGGAGGGTGGAAT	50.0	50.0	58.0	57.9	1573 bp	0.5	59.5
ndhI_p5	CGTTTATGGCTCCCACGTAA	TGTACTTGGGAGGGTGGAAT	50.0	50.0	58.0	57.9	1483 bp	0.5	59.5