Hotspot

ndhG

SSC · 125155-125685 · 531 bp

Back to Saururus

Pi 0.429

MarkerSeek score 75.40

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 161
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CTCATGAGAAACGGCCAAGA	AACTGCGTCGAGTATTGTCC	50.0	50.0	57.9	58.0	1863 bp	0.5	59.1
ndhG_p2	CTCATGAGAAACGGCCAAGA	CTGCGTCGAGTATTGTCCAA	50.0	50.0	57.9	58.0	1861 bp	0.5	59.1
ndhG_p3	CTCATGAGAAACGGCCAAGA	TTTTGTGGTAACTGCGTCGA	50.0	45.0	57.9	58.1	1872 bp	0.5	58.7
ndhG_p4	ATCTGCTCATGAGAAACGGC	AACTGCGTCGAGTATTGTCC	50.0	50.0	58.3	58.0	1868 bp	0.5	57.2
ndhG_p5	ATCTGCTCATGAGAAACGGC	CTGCGTCGAGTATTGTCCAA	50.0	50.0	58.3	58.0	1866 bp	0.5	57.2