Hotspot

ndhD

SSC · 122405-123910 · 1506 bp

Back to Saururus

Pi 0.487

MarkerSeek score 80.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 604
pi: 0.487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGTGTGAATTCGGCAATTG	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.0	58.0	2111 bp	0.5	59.7
ndhD_p2	GTTTGCAGGGTGTGAATTCG	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.9	58.0	2118 bp	0.5	59.4
ndhD_p3	TTACTTGGCGGGGTACAGTA	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.1	58.0	2404 bp	0.5	59.2
ndhD_p4	GGGTGTGAATTCGGCAATTG	TCTTGGCCGTTTCTCATGAG	50.0	50.0	58.0	57.9	2425 bp	0.5	59.2
ndhD_p5	AGGGTGTGAATTCGGCAATT	AGATGTGAATCCGCTTGTCC	45.0	50.0	58.1	58.0	2112 bp	0.5	59.1