Hotspot

ccsA

SSC · 121160-122131 · 972 bp

Back to Saururus

Pi 0.491

MarkerSeek score 82.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 428
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCCTAATCGTCCCGGATTTT	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.0	58.1	2012 bp	0.5	59.1
ccsA_p2	GCCTAATCGTCCCGGATTTT	TTTGTAGATTCTGGGCCACG	50.0	50.0	58.0	57.9	1809 bp	0.5	58.8
ccsA_p3	GCCTAATCGTCCCGGATTTT	CATTGCCAGGAATGAGTGGT	50.0	50.0	58.0	58.2	2009 bp	0.5	58.5
ccsA_p4	TAGACACGCTGCTCTTAGGA	TTGCATTGCCAGGAATGAGT	50.0	45.0	57.9	58.1	1784 bp	0.5	58.4
ccsA_p5	GCCTAATCGTCCCGGATTTT	CTTCTCTTGCATTGCCAGGA	50.0	50.0	58.0	58.2	2018 bp	0.5	58.3