Hotspot

ndhG-ndhI

SSC · 125686-126086 · 401 bp

Back to Saururus

Pi 0.439

MarkerSeek score 79.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 167
pi: 0.439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATGAAGTAACCCCATCCCCA	AACTGCGTCGAGTATTGTCC	50.0	50.0	58.0	58.0	894 bp	0.5	59.9
ndhG-ndhI_p2	ATGAAGTAACCCCATCCCCA	CTGCGTCGAGTATTGTCCAA	50.0	50.0	58.0	58.0	892 bp	0.5	59.9
ndhG-ndhI_p3	CGTTTATGGCTCCCACGTAA	AACTGCGTCGAGTATTGTCC	50.0	50.0	58.0	58.0	804 bp	0.5	59.8
ndhG-ndhI_p4	CGTTTATGGCTCCCACGTAA	CTGCGTCGAGTATTGTCCAA	50.0	50.0	58.0	58.0	802 bp	0.5	59.8
ndhG-ndhI_p5	CCCAACGGTCCAAAGAGAAA	AACTGCGTCGAGTATTGTCC	50.0	50.0	58.0	58.0	878 bp	0.5	59.7