Hotspot

trnL-UAG-ccsA

SSC · 121032-121159 · 128 bp

Back to Saururus

Pi 0.464

MarkerSeek score 76.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 128
variable_sites: 51
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GCCTAATCGTCCCGGATTTT	TTGGGGCTGTAATTGCACTT	50.0	45.0	58.0	58.0	730 bp	0.5	59.6
trnL-UAG-ccsA_p2	GCCTAATCGTCCCGGATTTT	TACTGTACCCCGCCAAGTAA	50.0	50.0	58.0	58.1	992 bp	0.5	59.2
trnL-UAG-ccsA_p3	GCCTAATCGTCCCGGATTTT	TCGTGCCCTTTTCTAACGAG	50.0	50.0	58.0	57.9	553 bp	0.5	59.1
trnL-UAG-ccsA_p4	GCCTAATCGTCCCGGATTTT	AGAAGTAGCTATCGTGCCCT	50.0	50.0	58.0	57.9	564 bp	0.5	59.1
trnL-UAG-ccsA_p5	TAGACACGCTGCTCTTAGGA	TTGGGGCTGTAATTGCACTT	50.0	45.0	57.9	58.0	502 bp	0.5	58.9