Hotspot

ndhF-rpl32

SSC · 118397-119662 · 1266 bp

Back to Saururus

Pi 0.458

MarkerSeek score 80.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1266
variable_sites: 474
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTAACTGGGAGTGGAACGA	TGCCGCCCAATATCCTTTTT	50.0	45.0	58.0	58.1	1407 bp	0.5	58.8
ndhF-rpl32_p2	AGTAACTGGGAGTGGAACGA	TTGCCGCCCAATATCCTTTT	50.0	45.0	58.0	58.1	1408 bp	0.5	58.8
ndhF-rpl32_p3	AGTAACTGGGAGTGGAACGA	TTTGCCGCCCAATATCCTTT	50.0	45.0	58.0	58.1	1409 bp	0.5	58.8
ndhF-rpl32_p4	AGTAACTGGGAGTGGAACGA	TTTTGCCGCCCAATATCCTT	50.0	45.0	58.0	58.1	1410 bp	0.5	58.8
ndhF-rpl32_p5	AGTAACTGGGAGTGGAACGA	TTTTTGCCGCCCAATATCCT	50.0	45.0	58.0	58.1	1411 bp	0.5	58.8