Hotspot

ycf4

LSC · 62086-62640 · 555 bp

Back to Saurauia

Pi 0.00541

MarkerSeek score 54.30

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 3
pi: 0.00541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TGGGCTATTCTCTCAATTCCA	AAACAGGAGGATTCTTGGCG	42.9	50.0	56.4	58.2	665 bp	1.0	58.2
ycf4_p2	TGGGCTATTCTCTCAATTCCA	CAGGAGGATTCTTGGCGTTT	42.9	50.0	56.4	58.2	662 bp	1.0	58.2
ycf4_p3	TGGGCTATTCTCTCAATTCCAA	AAACAGGAGGATTCTTGGCG	40.9	50.0	57.1	58.2	665 bp	1.0	56.1
ycf4_p4	TGGGCTATTCTCTCAATTCCAA	CAGGAGGATTCTTGGCGTTT	40.9	50.0	57.1	58.2	662 bp	1.0	56.1
ycf4_p5	TTGGGCTATTCTCTCAATTCCA	CAGGAGGATTCTTGGCGTTT	40.9	50.0	57.1	58.2	663 bp	1.0	56.1